Intrahospital supervised exercise training improves survival rate among hypertensive patients with COVID-19.

Among the people most affected by coronavirus disease 2019 (COVID-19) are those suffering from hypertension (HTN). However, pharmacological therapies for HTN are ineffective against COVID-19 progression and severity. It has been proposed that exercise training (EX) could be used as post-COVID treatment, which does not rule out the possible effects during hospitalization for COVID-19. Therefore, we aimed to determine the impact of supervised EX on HTN patients with COVID-19 during hospitalization. Among a total of 1,508 hospitalized patients with COVID-19 (confirmed by PCR), 439 subjects were classified as having HTN and were divided into two groups: EX (n = 201) and control (n = 238) groups. EX (3-4 times/wk during all hospitalizations) consisted of aerobic exercises (15-45 min; i.e., walking); breathing exercises (10-15 min) (i.e., diaphragmatic breathing, pursed-lip breathing, active abdominal contraction); and musculoskeletal exercises (8-10 sets of 12-15 repetitions/wk; lifting dumbbells, standing up and sitting, lumbar stabilization). Our data revealed that the EX (clinician: patient, 1:1 ratio) intervention was able to improve survival rates among controlled HTN patients with COVID-19 during their hospitalization when compared with the control group (chi-squared: 4.83; hazard ratio: 1.8; 95% CI: 1.117 to 2.899; P = 0.027). Multivariate logistic regression analysis revealed that EX was a prognostic marker (odds ratio: 0.449; 95% CI: 0.230-0.874; P = 0.018) along with sex and invasive and noninvasive mechanical ventilation. Our data showed that an intrahospital supervised EX program reduced the mortality rate among patients with HTN suffering from COVID-19 during their hospitalization.NEW & NOTEWORTHY In the present study, we found that exercise training improves the survival rate in hypertensive patients with COVID-19 during their hospitalization period. Our results provide strong evidence for the therapeutic efficacy of exercise training as a feasible approach to improving the outcomes of patients with COVID-19 who suffer from hypertension during their hospitalization.

Tenofovir Disoproxil Fumarate/Emtricitabine and Baricitinib for Patients at High Risk of Severe COVID-19: The PANCOVID Randomized Clinical Trial.

BACKGROUND: This study was designed to evaluate if patients with high risk for severe COVID-19 would benefit from treatment with TDF/FTC followed by baricitinib in case of hypoxemia and systemic inflammation. METHODS: PANCOVID is an open-label, double-randomized, phase 3 pragmatic clinical trial including adults with symptomatic COVID-19 with ≥ 2 comorbidities or older than 60 years conducted between 10 October 2020 and 23 September 2021. In the first randomization patients received TDF/FTC or not TDF/FTC. In the second randomization patients with room-air O2 saturation <95% and at least one increased inflammatory biomarker received baricitinib plus dexamethasone or dexamethasone alone. The primary endpoint was 28-day mortality. Main secondary endpoint was 28-day disease progression or critical care unit admission or mortality. The trial was stopped before reaching planned sample size due to the decrease in the number of cases and a mortality rate substantially lower than expected EudraCT registration number: 2020-001156-18. RESULTS: Of the 355 included participants 97% were hospitalized at baseline. Overall, 28-day mortality was 3.1%. The 28-day mortality relative risk (RR) for participants treated with TDF/FTC was 1.76 (95% CI 0.52-5.91; p= 0.379); it was 0.42 (95% CI 0.11-1.59; p= 0.201) for those treated with baricitinib. The 28-day RR for the main secondary combined endpoint for participants treated with TDF/FTC was 0.95 (95% CI 0.66-1.40; p = 0.774); it was 0.90 (95%CI 0.61-1.33; p = 0.687) for those treated with baricitinib. CONCLUSIONS: Our results do not suggest a beneficial effect of TDF/FTC; nevertheless, they are compatible with the beneficial effect of baricitinib already established by other clinical trials.

The psychological impact of the COVID-19 pandemic in Spain: A longitudinal study

Background: This study aims to longitudinally assess the psychological impact of the COVID-19 pandemic in the general Spanish population. It uses four assessment points: two weeks after the start of confinement, one month after, two months after, and one year after the first evaluation. Methods: Evaluations were conducted through an online survey, with a sample of 3,480 people at the first data collection and 1,041, 569, and 550 people at successive evaluation points. Depressive symptoms (PHQ-2), anxiety (GAD-2), post-traumatic stress (PCL-C-2), social support (EMAS), loneliness (UCLA-3), and discrimination (InDI-d) were evaluated. Results: Significant changes were found in the variables depression and anxiety with a greater presence of this kind of symptomatology after one year (p < .01). There were also significant changes in the variable social support, which showed a substantial reduction after one year (p < .001). Similarly, there were significant variations in the variable intersectional discrimination (p < .001), with greater levels of discrimination. The temporal models show no significant differences in terms of post-traumatic symptomatology (p = .12) or loneliness (p = .19). Conclusions: The pandemic had a negative impact on mental health and these effects were further exacerbated one year later. (PsycInfo Database Record (c) 2022 APA, all rights reserved) (Spanish) Antecedentes: el objetivo es evaluar el impacto psicologico de la pandemia generada por la COVID-19 en la poblacion general espanola longitudinalmente en cuatro momentos: tras dos semanas del inicio del confinamiento, al mes, a los dos meses y al ano. Metodo: las evaluaciones se realizaron mediante una encuesta online, se siguio a una muestra de 3.480 personas en la primera recogida de datos y de 1.041, 569 y 550 personas en los sucesivos momentos de evaluacion. Se evaluo la presencia de sintomas depresivos (PHQ-2), de ansiedad (GAD-2), de estres postraumatico (PCL-C-2), el apoyo social (EMAS), la soledad (UCLA-3) y la discriminacion (InDI-D). Resultados: se han producido cambios significativos en las variables de depresion y ansiedad con una presencia mayor de dicha sintomatologia al ano (p < .01), asi como en la variable de apoyo social, que muestra una reduccion significativa un ano despues (p < .001), y en la discriminacion interseccional, con una mayor discriminacion (p < .001). Los modelos temporales no muestran diferencias signi ficativas en cuanto a sintomatologia postraumatica (p = .12) ni soledad (p = .19). Conclusiones: la pandemia ha tenido un impacto negativo en la salud mental y estos efectos son todavia peores un ano despues. (PsycInfo Database Record (c) 2022 APA, all rights reserved)

Impacto del Covid-19 en la salud mental, uso y barreras en atención psicológica en España

La pandemia de Covid-19 ha tenido un grave impacto en la salud mental de la población, que además ha visto mermado su acceso a atención psicológica por diversas barreras. El objetivo del presente estudio es comparar el estado de salud mental de la población española al inicio del confinamiento (N = 3480) y 12 meses después (N = 550), y describir el uso de servicios de atención psicológica y sus principales barreras de acceso. Los participantes completaron una encuesta online. El 40% de la muestra reveló haber tenido problemas de salud mental desde el inicio de la pandemia, de los cuales el 23.4% señalaron haber recibido algún tipo de atención. La mayor parte refirieron no haber buscado atención porque pudieron manejar los problemas por sí mismos, sus problemas mejoraron solos, o no pudieron costearse tratamiento. La atención de los problemas de salud mental debe ser una prioridad para abordar el impacto psicológico del Covid-19.Alternate : The COVID-19 pandemic has had a serious impact on the mental health of the population, which also has been affected by various barriers to access to psychological care. The aim of the current study is to compare the mental health status of the Spanish population between the onset of confinement (N = 3480) to 12 months later (N = 550), and to describe the use of psychological care services and the main barriers to access. Study participants completed an online survey. Forty percent of the sample reported experiencing a mental health problem since the pandemic’s onset, of which 23.4% reported having received some kind of care. The majority reported not seeking care because they could handle the problems on their own, their problems got better on their own, or they could not afford treatment. The psychological effects of the pandemic have been increasing since its onset, so attention to mental health problems should be a priority.

LONGITUDINAL STUDY OF AGE-RELATED DIFFERENCES IN THE PSYCHOLOGICAL IMPACT OF CONFINEMENT AS A CONSEQUENCE OF COVID-19 IN A SPANISH SAMPLE

Se analizan las diferencias relacionadas con la edad en el impacto psicológico del confinamiento a consecuencia de la Covid-19 en una muestra española. Se realizó un estudio longitudinal (N= 1.041) mediante una encuesta online con dos mediciones: a las dos y cinco semanas de la declaración del estado de alarma en España. Se evaluaron mediante cuestionarios de detección los síntomas de trastorno de estrés postraumático (TEPT), ansiedad y depresión, bienestar espiritual y soledad percibida. Se calcularon las medias y sus intervalos de confianza (95%) para todas las variables del estudio, para los tres grupos de edad: 18-30, 31-59, 60-80. Para cada variable se calcularon modelos lineales mixtos con pendientes aleatorias (tiempo anidado a los sujetos). El impacto psicológico persiste a lo largo del tiempo, aumentando en algunas de las variables. El grupo de mayor edad muestra menor impacto y mayor bienestar. Presentan menos síntomas de depresión, ansiedad y TEPT y menos soledad. Estos resultados pueden explicarse por la mayor resiliencia de este grupo para recuperarse de situaciones adversas, y el mayor número de estrategias de afrontamiento.Alternate :This research aims to analyze age-related differences in the psychological impact of the Covid-19 confinement situation in a Spanish sample. A longitudinal study (N= 1,041) was conducted through an online survey with two measurements: at two and five weeks after the declaration of the alarm state in Spain. Post-traumatic stress disorder (PTSD), anxiety and depressive symptoms, spiritual well-being and perceived loneliness were evaluated by screening tests. Means and their confidence intervals (95%) were calculated for all variables in the study, for the three age groups: 18-30, 31-59, 60-80. Linear mixed models with random slopes (Time nested to Subjects) were calculated for each variable. The results indicate that the psychological impact caused by the pandemic persists over time, and even increases in some of the variables studied. The older age group (60-80 years) shows the least impact and the greatest well-being. They presented less depressive, anxious and PTSD symptoms and less loneliness. These results may be explained by the greater resilience of this group to recover from adverse situations, in addition to having a greater number of coping strategies.

Mental health consequences during the initial stage of the 2020 Coronavirus pandemic (COVID-19) in Spain.

The pandemic caused by Covid-19 has been an unprecedented social and health emergency worldwide. This is the first study in the scientific literature reporting the psychological impact of the Covid-19 outbreak in a sample of the Spanish population. A cross-sectional study was conducted through an online survey of 3480 people. The presence of depression, anxiety and post-traumatic stress disorder (PTSD) was evaluated with screening tests from 14 March. Sociodemographic and Covid-19-related data was collected. Additionally, spiritual well-being, loneliness, social support, discrimination and sense of belonging were assessed. Descriptive analyses were carried out and linear regression models compiled. The 18.7% of the sample revealed depressive, 21.6% anxiety and 15.8% PTSD symptoms. Being in the older age group, having economic stability and the belief that adequate information had been provided about the pandemic were negatively related to depression, anxiety and PTSD. However, female gender, previous diagnoses of mental health problems or neurological disorders, having symptoms associated with the virus, or those with a close relative infected were associated with greater symptomatology in all three variables. Predictive models revealed that the greatest protector for symptomatology was spiritual well-being, while loneliness was the strongest predictor of depression, anxiety and PTSD. The impact on our mental health caused by the pandemic and the measures adopted during the first weeks to deal with it are evident. In addition, it is possible to identify the need of greater psychological support in general and in certain particularly vulnerable groups.

The psychological impact of six weeks of lockdown as a consequence of COVID-19 and the importance of social support: A cross-cultural study comparing Spanish and Russian populations

Background: The COVID-19 pandemic has been an unprecedented social and health emergency worldwide. Cross-cultural research on mental health during this situation is needed to better understand its consequences. Objective: To evaluate the different psychological impacts of the crisis and lockdown situation during the first six weeks of COVID emergency measures in samples of the Spanish and Russian populations. Design: A cross-sectional study was conducted through an online survey (NSpain = 1041;NRussia = 743). The prevalence of loneliness, depression, anxiety, perceived discrimination (PD), internalized stigma (IS), and perceived social support (PSS) was evaluated. Chi-square tests and t-tests were administered. The Enter Method were used to identify the predictors of the mental health impact. Results: Differences were found between the Russian and the Spanish populations. While the degrees of anxiety and depression did not differ significantly, loneliness, the alienation dimension of IS, and PD were more pronounced in the Russian respondents. In Spain, the predictor of less negative impact was PSS from various sources, while in Russia we only found PSS from the family. Conclusion: Although in both countries the impact at the clinical level seemed to be similar, differences were found at the psychosocial level. Variables with a strong cultural component may be key to determining the means of alleviating the effects of the crisis, with PSS being a fundamental protective factor. More cross-sectional studies are needed to understand the impact of the pandemic in depth. (PsycInfo Database Record (c) 2022 APA, all rights reserved)

The Psychological Impact of the COVID-19 Pandemic in Spain: A Longitudinal Study.

BACKGROUND: This study aims to longitudinally assess the psychological impact of the COVID-19 pandemic in the general Spanish population. It uses four assessment points: two weeks after the start of confinement, one month after, two months after, and one year after the first evaluation. METHODS: Evaluations were conducted through an online survey, with a sample of 3,480 people at the first data collection and 1,041, 569, and 550 people at successive evaluation points. Depressive symptoms (PHQ-2), anxiety (GAD-2), post-traumatic stress (PCL-C-2), social support (EMAS), loneliness (UCLA-3), and discrimination (InDI-d) were evaluated. RESULTS: Significant changes were found in the variables depression and anxiety with a greater presence of this kind of symptomatology after one year (p < .01). There were also significant changes in the variable social support, which showed a substantial reduction after one year (p < .001). Similarly, there were significant variations in the variable intersectional discrimination (p < .001), with greater levels of discrimination. The temporal models show no significant differences in terms of post-traumatic symptomatology (p = .12) or loneliness (p = .19). CONCLUSIONS: The pandemic had a negative impact on mental health and these effects were further exacerbated one year later.

ESTUDIO LONGITUDINAL DE LA PERCEPCIÓN DE SOLEDAD DURANTE EL CONFINAMIENTO DERIVADO DE LA COVID-19 EN UNA MUESTRA DE POBLACIÓN ESPAÑOLA

To analyse the effect of the longitudinal measures, a mixed linear model (GLMM) was calculated for the solitude variable. The trend in loneliness scores is downward throughout the longitudinal study, decreasing the scores in the third evaluation significantly (Z(ti-t2) = 0.13, p = .045). Greater attention needs to be paid to loneliness in these situations, in addition to presenting attention to associated depressive symptomatology and measures to strengthen social support networks in these circumstances. (2020), con datos previos del UK Household Longitudinal Study y realizando una evaluación en abril de 2020 a 9748 adultos, encuentran similares medidas de soledad antes y después de la pandemia, mientras que Steptoe y Fancourt (2020), con datos previos provenientes también del UK Household Longitudinal Study con una evaluación posterior a 31064 personas en el proyecto del University College London COVID-19, encuentran que algunas personas que ya corrían el riesgo de estar solas como los adultos jóvenes de 18 a 30 años, las personas con bajos ingresos familiares y los adultos que vivían solos, experimentaron un mayor riesgo de soledad durante el confinamiento, añadiendo a estos resultados que el hecho de ser estudiante resultó ser un factor de riesgo diferencial más elevado de lo habitual durante el encierro.

Self-Compassion and Social Connectedness as Predictors of “Peace and Meaning” during Spain’s Initial COVID-19 Lockdown

The COVID-19 lockdown has had a massive psychological impact on mental health in the general population, with increases in anxiety, depression, and post-traumatic stress disorder. Spiritual well-being, specifically peace and meaning, has already been identified as one of the main protective factors for these disorders in the COVID-19 context. The aim of the present study is to identify facilitating elements for peace and meaning during the COVID-19 lockdown in Spain. Online surveys were used to obtain data from a sample of 3480 Spanish people. Self-compassion and social support were positively related with peace and meaning, while loneliness and perceived discrimination were negatively related. The model for peace and meaning was statistically significant, explaining 47% of the variance. The significant variables were self-kindness, family support, mindfulness, and sense of belonging having a positive association and loneliness a negative one.

Serial co-expression analysis of host factors from SARS-CoV viruses highly converges with former high-throughput screenings and proposes key regulators.

The current genomics era is bringing an unprecedented growth in the amount of gene expression data, only comparable to the exponential growth of sequences in databases during the last decades. This data allow the design of secondary analyses that take advantage of this information to create new knowledge. One of these feasible analyses is the evaluation of the expression level for a gene through a series of different conditions or cell types. Based on this idea, we have developed Automatic and Serial Analysis of CO-expression, which performs expression profiles for a given gene along hundreds of heterogeneous and normalized transcriptomics experiments and discover other genes that show either a similar or an inverse behavior. It might help to discover co-regulated genes, and common transcriptional regulators in any biological model. The present severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is an opportunity to test this novel approach due to the wealth of data that are being generated, which could be used for validating results. Thus, we have identified 35 host factors in the literature putatively involved in the infectious cycle of SARS-CoV viruses and searched for genes tightly co-expressed with them. We have found 1899 co-expressed genes whose assigned functions are strongly related to viral cycles. Moreover, this set of genes heavily overlaps with those identified by former laboratory high-throughput screenings (with P-value near 0). Our results reveal a series of common regulators, involved in immune and inflammatory responses that might be key virus targets to induce the coordinated expression of SARS-CoV-2 host factors.

Impact on polytrauma patient prehospital care during the first wave of the COVID-19 pandemic: a cross-sectional study.

BACKGROUND: The extraordinary situation caused by the onset of COVID-19 has meant that at prehospital level, the number of treatments, profile and time taken to respond for treating time-dependent pathologies has been greatly affected. However, it is not known whether the prehospital profile of polytrauma patients (PTP) has been affected. OBJECTIVE: To determine differences in the epidemiological characteristics and the clinical variables of prehospital polytrauma patients during the first wave of the COVID-19 pandemic in Catalonia. METHODOLOGY: Analytical cross-sectional study. The number of prehospital activations and the clinical and epidemiological characteristics of polytrauma patients attended by the Emergency Medical System (EMS) of Catalonia, were compared for the period between 15 February and 15 May 2020 and the same period in the previous year. Priorities 0 and 1 are assigned to the most severely injured patients. An analysis was conducted using logistic regression and nonparametric tests. RESULTS: 3023 patients were included. During the 2019 study period, 2045 (67.6%) patients were treated; however, during the pandemic period, 978 (32.4%) patients were treated, representing a 52% decrease (p = 0.002). The percentage of patients presenting priority 1 was higher during the pandemic period [240 (11.7%) vs 146 (14.9%), p = 0.032]. The percentage of priority 0 and 1 patients attended by a basic life support unit increased [201 (9.8%) vs 133 (13.6%), p = 0.006]. The number of traffic accidents decreased from 1211 (59.2%) to 522 (53.4%) and pedestrian-vehicle collisions fell from 249 (12.2%) to 92 (9.4%). Regarding weapon-related injuries and burns, there was an increase in the number of cases [43 (2.1%) vs 41 (4.2%), and 15 (0.7%) vs 22 (2.2%), p = 0.002 and p < 0.001, respectively]. Hospital mortality remained unchanged (3.9%). CONCLUSIONS: During the first wave of the pandemic, the number of polytrauma patients decreased and there was a change in the profile of severity and type of accident.

The Role of the Sense of Belonging During the Alarm Situation and Return to the New Normality of the 2020 Coronavirus Pandemic (COVID-19) in Spain.

In this study we intend to understand the impact of the COVID-19 crisis and the subsequent stay-at-home orders, on the Spanish population's sense of belonging at three moments in time: at the beginning of the lockdown, after one month of lockdown and with the return to the "new normality". A cross-sectional study was conducted through an online survey (N0 = 3480; N1 = 1041; N2 = 569). The sense of belonging was evaluated by means of four Likert-type items. These questions included membership in different groups: work/studies, friends, family and neighborhood or community. Sociodemographic and COVID-19-related data were collected. Additionally, mental health, spiritual well-being, loneliness, social support and discrimination were assessed. Descriptive analyses were carried out and linear regression models compiled. The sense of belonging increased significantly during confinement, dropping dramatically with the start of the return to the "new normality" process. The only variable that showed interaction with time and sense of belonging was discrimination. Work condition (not working providing the lowest sense of belonging scores), social support from friends and loneliness were the main predictors of the sense of belonging. The impact caused by the pandemic and the actions adopted during the first weeks regarding the sense of belonging is evident. It has been a key variable in dealing with COVID-19. Actions are now needed to increase our sense of belonging to face the post-epidemic crisis and avoid a greater impact in other areas.

Promoción de salud en centros de personas mayores ante el coronavirus mediante el uso de títeres

El confinamiento como medida preventiva ante la COVID19 ha supuesto un alto coste emocional y psicológico a la población en general y especialmente a las personas mayores. Resulta relevante la promoción de factores asociados a la resiliencia, con objeto de impulsar una gestión emocional y un afrontamiento positivo ante la situación presentada, que permitan generar condiciones afectivas y conductuales favorables para escenarios de futuro entre los miembros de la comunidad que constituyen los centros de mayores. Los recursos expresivos y las técnicas artísticas ofrecen procesos y resultados que fortalecen los factores resilientes. Ofrecemos la experiencia de trabajo con títeres empleados como objeto intermediario orientados a la promoción de la resiliencia y del clima relacional existente en los centros de mayores por motivo del confinamiento. Se toma en consideración la importancia del proceso de elaboración, los resultados obtenidos y la difusión de ambos aspectos en ámbitos externos a los centros de mayores. Se trataba de poner en valor el desarrollo de la actividad y el uso del títere como una herramienta de comunicación tanto a nivel interno (mayor-mayor y mayor-cuidador) como a nivel externo, en la relación establecida con colectivos y agentes externos a los centros de mayores.Alternate abstract: Confinement as a preventive measure against COVID19 has entailed a high emotional and psychological cost to the general population and especially to the elderly. The promotion of factors associated with resilience is relevant, in order to promote emotional management and positive coping in the face of the situation presented, which allow generating favorable affective and behavioral conditions for future scenarios among the members of the community that constitute the elderly centers. Expressive resources and artistic techniques offer processes and results that strengthen resilient factors. We offer the experience of working with puppets used as an intermediary object aimed at promoting resilience and the relational climate that exists in centers for the elderly due to confinement. The importance of the elaboration process, the results obtained, as well as the dissemination of both aspects in areas outside the centers for the elderly are taken into consideration, both in what speaks of the enhancement of the elderly in the development of the activity undertaken, such as with regard to the use of the puppet as a communication tool already internally between the elderly and their caregivers, as in the relationship established with groups and agents external to the elderly centers.

Effects of COVID-19 lockdown on perceived discrimination and internalized stigma in people with previous mental disorder diagnoses in Spain.

COVID-19 disease control efforts and consequences are likely to be complicated by the impact of fear and stigmatization of the novel coronavirus. These complications may also worsen due to the potential compounding of COVID-19 related stigma with stigmatization associated with previously diagnosed conditions. This exploratory study analyzes the experiences of the stigma associated with COVID-19 lockdown in people with different previous diagnoses 2 and 5 weeks after the beginning of the state of emergency in Spain. Gender and age were controlled as covariables and 1,052 people participated in the study. The diagnosis groups were: Psychiatry and mental health (n = 71), Cardiovascular disease (n = 42), Neurological disease (n = 23), Lung disease (n = 53), and No diagnosis (n = 863). The instruments used to measure the stigma were the Intersectional Day-to-Day Discrimination Index and two items of the Internalized Stigma of Mental Illness scale. Analysis of covariance of repeated measures and analysis of variance, including Scheffe's post hoc test, were performed. We found significant differences in stigma among the first and second evaluation. Regarding the previous diagnosis, no differences were found at Time 1, but significant differences were found at Time 2, with those having a previous psychiatric or mental health diagnosis reporting higher levels of perceived discrimination and internalized stigmatization. Our results suggest that people with previous stigmatizing conditions might be more vulnerable to experiencing stigma in a confinement situation. In order to reduce the burden of the COVID-19 stigma, health interventions should also consider the resulting intersection of effects on internalized stigma and perceived discrimination. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

A DNA intercalating dye-based RT-qPCR alternative to diagnose SARS-CoV-2.

Early detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proven crucial during the efforts to mitigate the effects of the COVID-19 pandemic. Several diagnostic methods have emerged in the past few months, each with different shortcomings and limitations. The current gold standard, RT-qPCR using fluorescent probes, relies on demanding equipment requirements plus the high costs of the probes and specific reaction mixes. To broaden the possibilities of reagents and thermocyclers that could be allocated towards this task, we have optimized an alternative strategy for RT-qPCR diagnosis. This is based on a widely used DNA-intercalating dye and can be implemented with several different qPCR reagents and instruments. Remarkably, the proposed qPCR method performs similarly to the broadly used TaqMan-based detection, in terms of specificity and sensitivity, thus representing a reliable tool. We think that, through enabling the use of vast range of thermocycler models and laboratory facilities for SARS-CoV-2 diagnosis, the alternative proposed here can increase dramatically the testing capability, especially in countries with limited access to costly technology and reagents.

Symptomatic Common Carotid Free-Floating Thrombus in a COVID-19 Patient, Case Report and Literature Review.

Carotid free-floating thrombus is an uncommon entity that usually presents with neurologic symptoms. Crescendo transient ischemic attack is an accepted indication for urgent carotid endarterectomy. COVID-19 is associated with severe thromboembolic complications. We report the case of a 61-year-old man who developed, 2 weeks after the diagnosis of COVID-19, crescendo transient ischemic attack, complicating a large intraluminal floating thrombus within the right common carotid artery. A carotid thromboendarterectomy under local anesthesia, with patch closure was immediately performed without complications. We conducted a literature review to identify cases of common carotid artery thrombus related to COVID-19. Carotid free-floating thrombus in the common carotid artery is exceptional. However, since the beginning of the COVID-19 pandemic, 15 cases have been published.

Evolution of intersectional perceived discrimination and internalized stigma during COVID-19 lockdown among the general population in Spain.

BACKGROUND: Stigma and discrimination have been associated with different diseases and pandemics, with negative consequences for the people who suffered them and for their communities. Currently, COVID-19 has become a new source of stigmatization. AIMS: The aim of the present study is to analyze longitudinally the evolution of intersectional perceived discrimination and internalized stigma among the general population of Spain, at three points in time throughout the confinement. METHOD: Participants completed an online survey. RESULTS: Results show an increase in both variables from the first to the second evaluation, and a slight decrease from the second to the third evaluation. Moreover, these changes are explained by depression, anxiety and family support. CONCLUSIONS: These findings indicate the factors that need to be considered to reduce the perception of discrimination and the internalization of stigma, and their detrimental consequences, during an especially stressful event such as the current pandemic outbreak.

Mental Health Consequences of the Coronavirus 2020 Pandemic (COVID-19) in Spain. A Longitudinal Study.

Background: Covid-19 remains a pandemic that most countries in the world are still dealing with. This is study aims to report the psychological impact of Covid-19 over time on the Spanish population. Methods: A longitudinal study (N = 1041) was carried out with two measurements: after 2 and 5 weeks starting from the declaration of the state of emergency in Spain. The presence of depressive symptoms, anxiety, and posttraumatic stress disease (PTSD) was evaluated by means of screening tests. Sociodemographic data, variables about Covid-19, loneliness, spiritual well-being, social support, discrimination, and a sense of belonging were collected. Results: The data showed how depressive symptomatology increased significantly over time, while anxiety and PTSD did not show statistically significant changes. Spiritual well-being and loneliness were the main predictors of psychological impact. A younger age was a significant predictor of depression and anxiety, while female gender was associated with anxiety and PTSD. Conclusions: The impact of the pandemic is sustained over time, even increasing in depression, and vulnerable groups that need greater psychological health support could be identified.